ACELL September 46/3
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چکیده
Stutzin, Andrés, Rubén Torres, Macarena Oporto, Patricio Pacheco, Ana Luisa Eguiguren, L. Pablo Cid, and Francisco V. Sepúlveda. Separate taurine and chloride efflux pathways activated during regulatory volume decrease. Am. J. Physiol. 277 (Cell Physiol. 46): C392–C402, 1999.—Organic osmolyte and halide permeability pathways activated in epithelial HeLa cells by cell swelling were studied by radiotracer efflux techniques and single-cell volume measurements. The replacement of extracellular Cl2 by anions that are more permeant through the volume-activated Cl2 channel, as indicated by electrophysiological measurements, significantly decreased taurine efflux. In the presence of less-permeant anions, an increase in taurine efflux was observed. Simultaneous measurement of the 125I, used as a tracer for Cl2, and [3H]taurine efflux showed that the time courses for the two effluxes differed. In Cl2-rich medium the increase in I2 efflux was transient, whereas that for taurine was sustained. Osmosensitive Cl2 conductance, assessed by measuring changes in cell volume, increased rapidly after hypotonic shock. The influx of taurine was able to counteract Cl2 conductance-dependent cell shrinkage but only ,4 min after triggering cell swelling. This taurine-induced effect was blocked by DIDS. Differences in anion sensitivity, the time course of activation, and sensitivity to DIDS suggest that the main cell swelling-activated permeability pathways for taurine and Cl2 are separate.
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ACELL September 46/3
Dodd, J. S., J. A. Raleigh, and T. S. Gross. Osteocyte hypoxia: a novel mechanotransduction pathway. Am. J. Physiol. 277 (Cell Physiol. 46): C598–C602, 1999.—Bone is a unique tissue in which to examine mechanotransduction due to its essential role in weight bearing. Within bone, the osteocyte is an ideal cellular mechanotransducer candidate. Because osteocytes reside distant from the blood supp...
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Zeiske, Wolfgang, Ilse Smets, Marcel Ameloot, Paul Steels, and Willy Van Driessche. Intracellular pH shifts in cultured kidney (A6) cells: effects on apical Na1 transport. Am. J. Physiol. 277 (Cell Physiol. 46): C469–C479, 1999.—We report, for the epithelial Na1 channel (ENaC) in A6 cells, the modulation by cell pH (pHc) of the transepithelial Na1 current (INa), the current through the individu...
متن کاملACELL September 46/3
Shepard, Allan R., and James L. Rae. Electrically silent potassium channel subunits from human lens epithelium. Am. J. Physiol. 277 (Cell Physiol. 46): C412–C424, 1999.—We describe the cloning and characterization of the first human members, hKv9.1 and hKv9.3, of the electrically silent delayed-rectifying-like K1 channel subfamily. Their modulatory effects on the electrically active subfamily m...
متن کاملACELL September 46/3
Stevens, Randel J., Jeffery S. Weinert, and Nelson G. Publicover. Visualization of origins and propagation of excitation in canine gastric smooth muscle. Am. J. Physiol. 277 (Cell Physiol. 46): C448–C460, 1999.—The origin and spread of excitation were visualized with fluo 3 fluorescence in tissues isolated from canine gastric antrum. Sheets of circular muscle (5 3 6 mm) had at least 1 (30%) and...
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JOHN M. PARK,1 ROSALYN M. ADAM,1 CRAIG A. PETERS,1 PAUL D. GUTHRIE,1 ZIJIE SUN,2 MICHAEL KLAGSBRUN,3 AND MICHAEL R. FREEMAN3 1Urologic Laboratory, Department of Urology, 3Laboratory for Surgical Research, Children’s Hospital, and Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115; and 2Departments of Surgery and Genetics, Stanford University School of Medicine, Stanford,...
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